Journal: Oncology Research

Article Title: miR-4262 Promotes Proliferation and Invasion of Human Breast Cancer Cells Through Directly Targeting KLF6 and KLF15

doi: 10.3727/096504016X14732514133203

Figure Lengend Snippet: miR-4262 was significantly upregulated in human breast cancer tissues and cell lines. Breast cancer tissues and paired adjacent noncancerous tissues were sampled from 48 breast cancer patients. The expression levels of miR-4262 were detected in these tissues with real-time qPCR. Then the expression levels of miR-4262 were detected in the human normal breast epithelial cell MCF-10A and five human breast cancer cell lines including MDA-MB-231, MDA-MB-468, MDA-MB-435, SKBR3, and MCF-7. (A) miR-4262 was significantly upregulated in breast cancer tissues. (B) miR-4262 was significantly elevated in breast cancer cell lines. ** p < 0.01, *** p < 0.001 versus MCF-10A.

Article Snippet: The primers of miR-4262 and U6 RNA (internal reference) were designed and produced by Ribobio Co., Ltd. PCR amplification cycles were performed using the SYBR Premix Ex Taq II Kit (Invitrogen).

Techniques: Expressing

Journal: Oncology Research

Article Title: miR-4262 Promotes Proliferation and Invasion of Human Breast Cancer Cells Through Directly Targeting KLF6 and KLF15

doi: 10.3727/096504016X14732514133203

Figure Lengend Snippet: miR-4262 mimic transfection promoted proliferation and invasion of MDA-MB-231 and MCF-7 breast cancer cell lines. The negative control mimic (NC) or miR-4262 mimic was transfected into MDA-MB-231 and MCF-7 human breast cancer cell lines. After incubation for 48 h, miR-4262 expression was detected with real-time qPCR, cell proliferation was detected with the CCK-8 method, and cell invasion was detected with the Transwell invasion assay. (A) miR-4262 expression was increased greatly by the miR-4262 mimic transfection. (B, C) The miR-4262 mimic significantly promoted the proliferation of MDA-MB-231 and MCF-7 cells. (D) The miR-4262 mimic significantly enhanced the invasion of MDA-MB-231 and MCF-7 cells. * p < 0.05, * p < 0.01 versus NC.

Article Snippet: The primers of miR-4262 and U6 RNA (internal reference) were designed and produced by Ribobio Co., Ltd. PCR amplification cycles were performed using the SYBR Premix Ex Taq II Kit (Invitrogen).

Techniques: Transfection, Negative Control, Incubation, Expressing, CCK-8 Assay, Transwell Invasion Assay

Journal: Oncology Research

Article Title: miR-4262 Promotes Proliferation and Invasion of Human Breast Cancer Cells Through Directly Targeting KLF6 and KLF15

doi: 10.3727/096504016X14732514133203

Figure Lengend Snippet: miR-4262 antagomir increased proliferation and invasion of MDA-MB-231 and MCF-7 cells. The control or antagomir miR-4262 oligo was transfected into MDA-MB-231 and MCF-7 cells. After incubation for 48 h, miR-4262 expression, cell proliferation, and cell invasion were detected. (A) The miR-4262 level was sharply decreased by the anta-miR-4262 transfection. (B, C) The anta-miR-4262 suppressed the proliferation of MDA-MB-231 and MCF-7 cells. (D) The anta-miR-4262 suppressed the invasion of MDA-MB-231 and MCF-7 cells. * p < 0.05, ** p < 0.01 versus Ctrl-anta.

Article Snippet: The primers of miR-4262 and U6 RNA (internal reference) were designed and produced by Ribobio Co., Ltd. PCR amplification cycles were performed using the SYBR Premix Ex Taq II Kit (Invitrogen).

Techniques: Transfection, Incubation, Expressing

Journal: Oncology Research

Article Title: miR-4262 Promotes Proliferation and Invasion of Human Breast Cancer Cells Through Directly Targeting KLF6 and KLF15

doi: 10.3727/096504016X14732514133203

Figure Lengend Snippet: miR-4262 targeted KLF6 and KLF15 mRNA at the 3′-UTR region. (A, B) Outputs of the miRDB online servers on the binding of miR-4262 with the 3′-UTR region of KLF6 and KLF15 mRNAs. (C, D) Validation of the binding of miR-4262 with KLF6 and KLF15 mRNAs by the 3′-UTR luciferase reporter gene assay. *** p < 0.001 versus control.

Article Snippet: The primers of miR-4262 and U6 RNA (internal reference) were designed and produced by Ribobio Co., Ltd. PCR amplification cycles were performed using the SYBR Premix Ex Taq II Kit (Invitrogen).

Techniques: Binding Assay, Luciferase, Reporter Gene Assay

Journal: Oncology Research

Article Title: miR-4262 Promotes Proliferation and Invasion of Human Breast Cancer Cells Through Directly Targeting KLF6 and KLF15

doi: 10.3727/096504016X14732514133203

Figure Lengend Snippet: miR-4262 negatively regulated the protein levels of KLF6 and KLF15. The MDA-MB-231 was transfected with 60 nM NC mimic, 60 nM miR-4262 mimic, 60 nM miR-4262 mimic plus 2 μg of pcDNA3.1 empty vector, or 60 nM miR-4262 mimic plus 2 μg of pcDNA-KLF6 and 2 μg of pcDNA-KLF15. After incubation for 48 h, Western blotting was used to detect the protein levels of KLF6, KLF15, EGFR, pEGFR, and CCND1. (A) The blots of KLF6, KLF15, EGFR, pEGFR, and CCND1. (B) Statistical analysis for (A). * p < 0.05, ** p < 0.01 versus NC.

Article Snippet: The primers of miR-4262 and U6 RNA (internal reference) were designed and produced by Ribobio Co., Ltd. PCR amplification cycles were performed using the SYBR Premix Ex Taq II Kit (Invitrogen).

Techniques: Transfection, Plasmid Preparation, Incubation, Western Blot